Protein Purification: Principles and Practice
- R. Skopes
The immunodiffusion method proposed by N. I. Khramkova and G. I. Abelev, which is a modification of Ouchterlony's test, is intended for semiquantitative determination of protein antigens. It is characterized by a relatively high sensitivity and absolute specificity.and permits objective recording of the results without separating the test antigens from the protein mixtures. In addition, it allows the content of immunoreactive molecules to be determined in purified protein preparations and can thus be used as a reference method in evaluating the nativity of the proteins obtained. In this comparative study, protein preparations of animal and plant origin were analyzed using spectrophotometric techniques and the immunodiffusion method to determine protein concentrations in solution.