Evaluation of the microbiota of primary endodontic infections using checkerboard DNA-DNA hybridization.

@article{Sassone2007EvaluationOT,
  title={Evaluation of the microbiota of primary endodontic infections using checkerboard DNA-DNA hybridization.},
  author={Luciana Moura Sassone and R. Fidel and Luciene Cristina de Figueiredo and Sandra Rivera Fidel and M Faveri and Magda Feres},
  journal={Oral microbiology and immunology},
  year={2007},
  volume={22 6},
  pages={390-7}
}
BACKGROUND/AIMS The aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp. METHODS Samples were collected from the root canals using #15 Hedströen-type files and two sterile paper points, which were introduced 1 mm short of the apical foramen. The presence, levels, and proportions of 40 different bacterial species in each sample were determined using DNA probes and checkerboard DNA… CONTINUE READING