Evaluation of the luciferase assay-based in vitro elicitation test for serum IgE.

@article{Nakamura2012EvaluationOT,
  title={Evaluation of the luciferase assay-based in vitro elicitation test for serum IgE.},
  author={Ryosuke Nakamura and Ayano Ishiwatari and Masakazu Higuchi and Yoshimi Uchida and Rika Nakamura and Hiroshi Kawakami and Atsuo Urisu and R Teshima},
  journal={Allergology international : official journal of the Japanese Society of Allergology},
  year={2012},
  volume={61 3},
  pages={431-7}
}
BACKGROUND An in vitro elicitation test employing human high-affinity IgE receptor-expressing rat mast cell lines appears to be a useful method for measuring mast cell activation using a patient's IgE and an allergen; however, such cell lines are sensitive to human complements in the serum. We have recently developed a new luciferase-reporting mast cell line (RS-ATL8) to detect IgE crosslinking-induced luciferase expression (EXiLE) with relatively low quantities of serum IgE. METHODS A total… CONTINUE READING

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