Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.

@article{Boue2003EvaluationOT,
  title={Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.},
  author={Stephen M. Boue and Thomas E. Wiese and S. Nehls and Matthew E. Burow and Steven G. Elliott and Carol H. Carter-Wientjes and Betty Y. Shih and J A McLachlan and Thomas E. Cleveland},
  journal={Journal of agricultural and food chemistry},
  year={2003},
  volume={51 8},
  pages={
          2193-9
        }
}
Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic… 

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References

SHOWING 1-10 OF 43 REFERENCES

Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms.

Genistein was found to be the most effective of four red clover isoflavones tested in the above in vitro assays, suggesting a potential use for some dietary supplements, ingested by human beings, in the treatment of menopausal symptoms.

Estrogen and Progestin Bioactivity of Foods, Herbs, and Spices

  • D. ZavaC. DollbaumM. Blen
  • Medicine, Biology
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1998
It is demonstrated that many of the commonly consumed foods, herbs, and spices contain phytoestrogens and phytoprogestins that act as agonists and antagonists in Vivo.

Estrogenic and Antiestrogenic Activities of FlavonoidPhytochemicals Through Estrogen ReceptorBinding-Dependent and -Independent Mechanisms

It is concluded thatAntiestrogenic activities of flavonoid phytochemicals may occur through ER binding-dependent and -independent mechanisms and that the binding-independent antiestrogen activity of certain flavonoids is biologically significant in regulation of breast cancer cell proliferation.

Detection of weak estrogenic flavonoids using a recombinant yeast strain and a modified MCF7 cell proliferation assay.

Evidence is provided that several of the flavo-estrogens possess estrogenic properties comparable in activity to the well-established isoflavonoid estrogens.

Commonly occurring plant flavonoids have estrogenic activity.

Evidence is presented to show that selected hydroxylated flavonoids interact directly with the estrogen receptor, based on their ability to compete for the binding of 17 beta-[3H]estradiol to the receptor in cell-free extracts.

The chemopreventive properties of soy isoflavonoids in animal models of breast cancer

  • S. Barnes
  • Biology
    Breast Cancer Research and Treatment
  • 2004
Future studies on the anticancer potential of soy isoflavonoids should examine their interaction with other phytochemical components of soybeans and exploit newly developed animal models of breast cancer in which specific genes have been activated or inactivated.

Phytoestrogen interaction with estrogen receptors in human breast cancer cells.

In sum, phytoestrogens interact with the estrogen receptors of human breast cancer cells in culture and, therefore, may affect estrogen-mediated events in these cells.

Interaction of Estrogenic Chemicals and Phytoestrogens with Estrogen Receptor β.

The estrogenic activity of environmental chemicals and phytoestrogens in competition binding assays with ERα or ERβ protein, and in a transient gene expression assay using cells in which an acute estrogenic response is created by cotransfecting cultures with recombinant human ERβ complementary DNA (cDNA) in the presence of an estrogen-dependent reporter plasmid are investigated.