Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.

@article{Logan2010EvaluationOT,
  title={Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.},
  author={Catriona Logan and Adele Habington and Gr{\'a}inne Lennon and Frank Cronin and Niamh O'Sullivan},
  journal={Diagnostic microbiology and infectious disease},
  year={2010},
  volume={68 4},
  pages={358-65}
}
A longitudinal study of 2099 sputa and throat swabs received from 183 pediatric cystic fibrosis patients over a 29-month period was used to evaluate the efficacy of real-time polymerase chain reaction (PCR) for the early detection of Pseudomonas aeruginosa as compared to microbiologic culture. Real-time PCR resulted in an increased number of specimens identified as P. aeruginosa positive. The sensitivity of culture was 82% (373/453) and of PCR was 93% (420/453) when considering both positive… CONTINUE READING

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