Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytilus edulis L. (Mollusca: Pelecypoda).

  title={Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytilus edulis L. (Mollusca: Pelecypoda).},
  author={J. T. Wilson and P. L. Pascoe and James M. Parry and David R. Dixon},
  journal={Mutation research},
  volume={399 1},
Influence of the SCGE protocol on the amount of basal DNA damage detected in the Mediterranean mussel, Mytilus galloprovincialis
The results suggest that the internal fluids of M. galloprovincialis may increase the basal levels of DNA SBs through oxidative and/or enzyme‐mediated pathways, and the SCGE protocol should be carefully considered when assessing DNA damage in these species.
In vitro application of the comet assay for aquatic genotoxicity: considering a primary culture versus a cell line.
  • I. Kamer, B. Rinkevich
  • Biology
    Toxicology in vitro : an international journal published in association with BIBRA
  • 2002
Comet assay in ecogenotoxicology: Applications in Mytilus sp.
The level of DNA damage and the frequency of micronuclei in haemolymph of freshwater messels Anodonta woodiana exposed to benzo[a]pyrene
The mussel A. woodiana-based assay to detect genotoxic potential of b[a]p and possibly other PAHs seems to have broad applications to aquatic toxicology and environmental monitoring.


Alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp
The results of the SCG procedure in bullheads and carp indicate that this assay is extremely sensitive and should be useful in detecting DNA damage caused by environmental contaminants.
Cytogenetic studies of PCB77 on brown trout (Salmo trutta fario) using the micronucleus test and the alkaline comet assay.
The DNA breakage and clastogenic potency of a planar PCB77 was determined in vivo in fish using the single cell gel electrophoresis or comet assay and the micronucleus test and in neither of the two tests used, were mutagenic effects due to PCB exposure observed.
The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment.
Cultured hepatocytes have been treated with either DMSO or dimethylnitrosamine (NDMA) for either 1 or 2 h and the cells assessed for DNA-damage using the single cell alkaline gel electrophoresis
Oxidative damage to DNA: do we have a reliable biomarker?
Using a revised DNA isolation procedure designed to decrease in vitro oxidation, results are obtained for 8-OHgua concentrations in human lymphocytes that are closure to the figures obtained by the comet assay.