Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytilus edulis L. (Mollusca: Pelecypoda).

@article{Wilson1998EvaluationOT,
  title={Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytilus edulis L. (Mollusca: Pelecypoda).},
  author={J. T. Wilson and P. L. Pascoe and James M. Parry and David R. Dixon},
  journal={Mutation research},
  year={1998},
  volume={399 1},
  pages={
          87-95
        }
}
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170 Citations
Highly Influential Citations
6
Background Citations
36
Methods Citations
28
Results Citations
6

170 Citations

Citation Type

Citation Type

Influence of the SCGE protocol on the amount of basal DNA damage detected in the Mediterranean mussel, Mytilus galloprovincialis
TLDR
The results suggest that the internal fluids of M. galloprovincialis may increase the basal levels of DNA SBs through oxidative and/or enzyme‐mediated pathways, and the SCGE protocol should be carefully considered when assessing DNA damage in these species.
An evaluation of the relative sensitivity of two marine bivalve mollusc species using the Comet assay.
Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis).
In vitro application of the comet assay for aquatic genotoxicity: considering a primary culture versus a cell line.
  • I. Kamer, B. Rinkevich
  • Biology
    Toxicology in vitro : an international journal published in association with BIBRA
  • 2002
Malathion Induced DNA Damage in Freshwater Fish, Labeo rohita (Hamilton, 1822) Using Alkaline Single Cell Gel Electrophoresis
Profenofos induced DNA damage in freshwater fish, Channa punctatus (Bloch) using alkaline single cell gel electrophoresis.
Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals.
Comet assay in ecogenotoxicology: Applications in Mytilus sp.
The level of DNA damage and the frequency of micronuclei in haemolymph of freshwater messels Anodonta woodiana exposed to benzo[a]pyrene
TLDR
The mussel A. woodiana-based assay to detect genotoxic potential of b[a]p and possibly other PAHs seems to have broad applications to aquatic toxicology and environmental monitoring.
Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments.
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References

SHOWING 1-10 OF 28 REFERENCES
Detection of DNA damage in individual cells from marine organisms using the single cell gel assay
Formation of DNA adducts in the gill tissue of Mytilus galloprovincialis treated with benzo[a]pyrene
Alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp
TLDR
The results of the SCG procedure in bullheads and carp indicate that this assay is extremely sensitive and should be useful in detecting DNA damage caused by environmental contaminants.
Cytogenetic studies of PCB77 on brown trout (Salmo trutta fario) using the micronucleus test and the alkaline comet assay.
TLDR
The DNA breakage and clastogenic potency of a planar PCB77 was determined in vivo in fish using the single cell gel electrophoresis or comet assay and the micronucleus test and in neither of the two tests used, were mutagenic effects due to PCB exposure observed.
The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment.
Cultured hepatocytes have been treated with either DMSO or dimethylnitrosamine (NDMA) for either 1 or 2 h and the cells assessed for DNA-damage using the single cell alkaline gel electrophoresis…
Contribution of apoptosis to observed dna damage in mussel cells
Induction of DNA strand breaks in rainbow trout Oncorhynchus mykiss under hypoxic and hyperoxic conditions
Oxidative damage to DNA: do we have a reliable biomarker?
TLDR
Using a revised DNA isolation procedure designed to decrease in vitro oxidation, results are obtained for 8-OHgua concentrations in human lymphocytes that are closure to the figures obtained by the comet assay.
An age-related increase in the basal level of DNA damage and DNA vulnerability to oxygen radicals in the individual hepatocytes of male F344 rats.
A simple technique for quantitation of low levels of DNA damage in individual cells.
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