Evaluation of the combined application of ethanol-fixed and formaldehyde-fixed neutrophil substrates for identifying atypical perinuclear antineutrophil cytoplasmic antibodies in inflammatory bowel disease.

@article{Papp2009EvaluationOT,
  title={Evaluation of the combined application of ethanol-fixed and formaldehyde-fixed neutrophil substrates for identifying atypical perinuclear antineutrophil cytoplasmic antibodies in inflammatory bowel disease.},
  author={Maria Papp and Istv{\'a}n Altorjay and Gabriella Lakos and Judit Tumpek and S{\'a}ndor Sipka and Tam{\'a}s Dinya and K{\'a}roly Palatka and G{\'a}bor G{\'a}bor Veres and Mikl{\'o}s Udvardy and Peter L Lakatos},
  journal={Clinical and vaccine immunology : CVI},
  year={2009},
  volume={16 4},
  pages={464-70}
}
No clear guidelines for indirect immunofluorescence (IIF) detection and interpretation of antineutrophil cytoplasmic antibodies (ANCA) have been proposed for inflammatory bowel diseases (IBD). We evaluated the reliability of the combined use of ethanol- and formalin-fixed neutrophil substrates to identify atypical perinuclear ANCA (P-ANCA) by IIF under routine laboratory circumstances. A total of 204 IBD patients were assessed with four different fluorescent substrates in two distinct… CONTINUE READING

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