Evaluation of the analytical sensitivity of a polymerase chain reaction assay for the detection of chicken infectious anemia virus in avian vaccines.

@article{Hermann2012EvaluationOT,
  title={Evaluation of the analytical sensitivity of a polymerase chain reaction assay for the detection of chicken infectious anemia virus in avian vaccines.},
  author={Joseph R Hermann and Danielle Mae Koski and Sharon I Taylor and Daniel Gatewood},
  journal={Biologicals : journal of the International Association of Biological Standardization},
  year={2012},
  volume={40 4},
  pages={266-9}
}
Chicken infectious anemia virus (CAV) is a ubiquitous pathogen of chickens causing significant disease in commercial flocks worldwide. During CAV outbreaks, the Center for Veterinary Biologics requires manufacturers of veterinary biologicals to test materials derived from infected flocks for extraneous CAV by polymerase chain reaction (PCR). The analytical sensitivity of a PCR assay for detection of CAV was determined and the applicability of a CAV DNA standard as a positive control for assay… CONTINUE READING

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