Evaluation of the alamarblue assay for adherent cell irradiation experiments.

@article{Zachari2014EvaluationOT,
  title={Evaluation of the alamarblue assay for adherent cell irradiation experiments.},
  author={Maria A Zachari and Panagiota S Chondrou and Stamatia E Pouliliou and Achilleas G Mitrakas and Ioannis M Abatzoglou and Christos E. Zois and Michael I. Koukourakis},
  journal={Dose-response : a publication of International Hormesis Society},
  year={2014},
  volume={12 2},
  pages={246-58}
}
The AlamarBlue assay is based on fluorometric detection of metabolic mitochondrial activity of cells. In this study, we determined the methodology for application of the assay to radiation response experiments in 96-well plates. AlamarBlue was added and its reduction measured 7 hours later. Selection of the initial number of plated cells was important so… CONTINUE READING