Evaluation of the Genetic Toxicity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells

Abstract

OBJECTIVES In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test. METHODS The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix). RESULTS Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method. CONCLUSION From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions.

DOI: 10.5491/SHAW.2011.2.1.17

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@inproceedings{Kim2011EvaluationOT, title={Evaluation of the Genetic Toxicity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells}, author={Soo-Jin Kim and Kyung-Taek Rim and Jong-Kyu Kim and Hyeon-Yeong Kim and Jeong-Sun Yang}, booktitle={Safety and health at work}, year={2011} }