Evaluation of the CaMAL2 promoter for regulated expression of genes in Candida albicans.

@article{Backen2000EvaluationOT,
  title={Evaluation of the CaMAL2 promoter for regulated expression of genes in Candida albicans.},
  author={Alison C Backen and Ian D. Broadbent and R W Fetherston and John Rosamond and Norbert Schnell and Michael J R Stark},
  journal={Yeast},
  year={2000},
  volume={16 12},
  pages={1121-9}
}
An expression vector (CIp10-MAL2p) for use in Candida albicans has been constructed in which a gene of interest can be placed under the control of the CaMAL2 maltase promoter and stably integrated at the CaRP10 locus. Using this vector to express the Candida URA3 gene from the CaMAL2 promoter, we have demonstrated tight regulation of CaURA3 expression by carbon source. Thus under conditions when the CaMAL2 promoter is not induced, expression of Candida URA3 was unable either to complement a C… CONTINUE READING
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