Evaluation of support materials for the immobilization of sulfate-reducing bacteria and methanogenic archaea.

  title={Evaluation of support materials for the immobilization of sulfate-reducing bacteria and methanogenic archaea.},
  author={A J Silva and Julia Sumiko Hirasawa and Maria Bernadete Am{\^a}ncio Varesche and Eug{\^e}nio Foresti and Marcelo Zaiat},
  volume={12 2},
This paper reports on the adhesion of sulfate-reducing bacteria (SRB) and methanogenic archaea on polyurethane foam (PU), vegetal carbon (VC), low-density polyethylene (PE) and alumina-based ceramics (CE). Anaerobic differential reactors fed with a sulfate-rich synthetic wastewater were used to evaluate the formation of a biofilm. The PU presented the highest specific biomass concentration throughout the experiment, achieving 872 mg TVS/g support, while 84 mg TVS/g support was the maximum value… Expand
Kinetic modeling and microbial assessment by fluorescent in situ hybridization in anaerobic sequencing batch biofilm reactors treating sulfate-rich wastewater
This paper reports the results of applying anaerobic sequencing batch biofilm reactors (AnSBBR) for treating sulfate-rich wastewater. The reactor was filled with polyurethane foam matrices or withExpand
Effects of bed materials on the performance of an anaerobic sequencing batch biofilm reactor treating domestic sewage.
PO was considered the most suitable material showing the best performance in terms of efficiency of solids and COD removal, while PE did not show a satisfactory performance, but an interesting microbial diversity was found on its surface. Expand
Microbial immobilization on synthetic polymers for wastewater treatment
Objective. Evaluate the ability of microbial immobilization of several synthetic polymers, and establish its performance during the start-up and operation of a hybrid biological reactor (HBR),Expand
Column experiments for immobilization of sulfate reducing babteria
In view of the difficulty in treating the bioleaching solutions, the selective precipitation of metals using H2S produced biologically by sulfate reducing bacteria (SRB) was put forward. In thisExpand
Application of horizontal-flow anaerobic immobilized biomass reactor for bioremediation of acid mine drainage.
Results showed that a maximal efficiency in the removal of sulfate and ethanol could only be obtained by reducing the applied sulfate load, and this strategy led to over 75% of chemical oxygen demand (COD) and sulfate removal. Expand
Preparation of immobilized sulfate reducing bacteria (SRB) granules for effective bioremediation of acid mine drainage and bacterial community analysis
Abstract Heavy metal-resistant immobilized sulfate-reducing bacteria (SRB) granules were prepared to treat acid mine drainage (AMD) containing high concentrations of multiple heavy metal ions usingExpand
Nitrate Reduction, Sulfate Reduction and Methanogenesis Interrelation in Fixed and Suspended Bed Batch Reactors
This study was focused on the interrelation among nitrate reduction, sulfate reduction and methanogenesis in fixed bed and suspended reactors. Dried stems of the cactus Opuntia imbricata were used asExpand
Full-scale anaerobic sequencing batch biofilm reactor for sulfate-rich wastewater treatment
This paper describes the performance and biofilm characteristics of a full-scale anaerobic sequencing batch biofilm reactor (ASBBR; 20 m 3 ) containing biomass immobilized on an inert supportExpand
Application of horizontal-fl ow anaerobic immobilized biomass reactor for bioremediation of acid mine drainage
The production of low-pH effluent with sulfate and metals is one of the biggest environmental concerns in the mining industry. The biological process for sulfate reduction has the potential to becomeExpand
Microbial Characterization of Methanogenic and Iron-reducing Consortium in Reactors with Polychlorinated Biphenyls
The relevant role of iron-reducing organisms and Chloroflexi, Geobacteraceae, and Firmicutes group in PCB metabolism is indicated. Expand


Influence of porosity and composition of supports on the methanogenic biofilm characteristics developed in a fixed bed anaerobic reactor.
This paper reports on the influence of the material porosity on the anaerobic biomass adhesion on four different inert matrices: polyurethane foam, PVC, refractory brick and special ceramic. TheExpand
Microbial colonization of polyurethane foam matrices in horizontal-flow anaerobic immobilized-sludge reactor
Abstract This paper presents the anaerobic biomass characterization and the bacterial framework inside polyurethane foam matrices taken from a horizontal-flow anaerobic immobilized-sludge (HAIS)Expand
Granulation and sludge bed stability in upflow anaerobic sludge bed reactors in relation to surface thermodynamics
Two types of structured granules were identified which had acidogens as solid-phase emulsifiers around a methanogenic association appeared to allow more stable reactor performance and decreasing the (gamma)(infLV) in the reactor by adding trace amounts of a surfactant increased reactor stability. Expand
Performance of horizontal-flow anaerobic immobilized sludge (HAIS) reactor treating synthetic substrate subjected to decreasing COD to sulfate ratios
The performance of a bench-scale horizontal-flow anaerobic immobilized sludge (HAIS) reactor treating a synthetic substrate under increasing influent sulfate (SO 4 2- ) concentrations wasExpand
Influence of the COD to sulphate ratio on the anaerobic organic matter degradation kinetics
The influence of the COD to sulphate ratio on the substrate consumption kinetic parameters was evaluated using a differential reactor filled with polyurethane foam matrices, taken from aExpand
Initial adhesion of methanogenic bacteria to polymers
SummaryAdhesion of four methanogenic bacteria on polymeric surfaces with different hydrophobicities was investigated using an adhesion test performed under anaerobic conditions.Methanosarcina mazeiExpand
Monitoring the development of anaerobic biofilms using fluorescent in situ hybridization and confocal laser scanning microscopy
The results showed that the modified Robbins Device together with the fluorescent in situ hybridization and confocal laser scanning microscopy are suitable tools to study anaerobic biofilm development in different kinds of support materials. Expand
Sulphate removal from industrial wastewater using a packed-bed anaerobic reactor
Abstract The feasibility of sulphate removal from sulphate-rich wastewater using an anaerobic fixed-bed reactor was investigated. The bioreactor was installed at a chemical industry producing organicExpand
Phylogenetic Oligodeoxynucleotide Probes for the Major Subclasses of Proteobacteria: Problems and Solutions
Based on comparative analyses of 16S and 23S ribosomal RNA sequences, sites specific for the alpha-, beta-, and gamma-subclasses of Proteobacteria are located and will be useful for determinative studies and for the in situ monitoring of population distribution and dynamics in microbial communities. Expand
Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations
Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA. Expand