Evaluation of subsampling and fixation procedures used for counting rumen protozoa.

@article{Dehority1984EvaluationOS,
  title={Evaluation of subsampling and fixation procedures used for counting rumen protozoa.},
  author={Burk A. Dehority},
  journal={Applied and environmental microbiology},
  year={1984},
  volume={48 1},
  pages={
          182-5
        }
}
Total numbers of protozoa can be significantly lower in rumen fluid than in whole rumen contents, depending on the time of sampling and the procedure used to separate the fluid and solid fractions. Moreover, generic distribution in rumen fluid was significantly affected in all cases tested. The percentage of Entodinium spp. increased, whereas percentages of Diplodinium spp. and Ophryoscolex spp. decreased. Microscopic observation of fresh and fixed rumen contents did not indicate any marked… CONTINUE READING

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Rumen ciliate protozoa of the sable antelope Hippotrogius niger

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