Evaluation of subsampling and fixation procedures used for counting rumen protozoa

@article{Dehority1984EvaluationOS,
  title={Evaluation of subsampling and fixation procedures used for counting rumen protozoa},
  author={Burk A. Dehority},
  journal={Applied and Environmental Microbiology},
  year={1984},
  volume={48},
  pages={182 - 185}
}
  • B. A. Dehority
  • Published 1 July 1984
  • Medicine, Biology
  • Applied and Environmental Microbiology
Total numbers of protozoa can be significantly lower in rumen fluid than in whole rumen contents, depending on the time of sampling and the procedure used to separate the fluid and solid fractions. Moreover, generic distribution in rumen fluid was significantly affected in all cases tested. The percentage of Entodinium spp. increased, whereas percentages of Diplodinium spp. and Ophryoscolex spp. decreased. Microscopic observation of fresh and fixed rumen contents did not indicate any marked… 

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  • B. A. Dehority
  • Biology, Medicine
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  • 1994
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Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR.

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Protozoários ruminais de novilhos de corte criados em pastagem tropical durante o período seco

The concentration average of the total and of small and medium protozoa declined significantly over the course of the dry season; however, alterations in large protozOA concentrations were not detected over the three periods evaluated.

Effects of Isotricha, Dasytricha, Entodinium, and total fauna on ruminal fermentation and duodenal flow in wethers fed different diets.

Entodinium was the most detrimental of ciliate protozoa species concerning protein nutrition of the host ruminant in wethers containing total fauna.

Sheep fed with banana leaf hay reduce ruminal protozoa population

The physicochemical characteristics of rumen fluid and the profile of the rumen protozoa populations were evaluated for sheep fed banana leaf hay in replacement of the Cynodon dactylon cv.

Improved in vitro procedure for maintaining stock cultures of three genera of rumen protozoa.

Although bacterial concentrations decreased when cultures were not fed for 2 d (over the weekend), they recovered quickly with subsequent daily feedings, and addition of streptomycin to the media had little effect on maintaining bacterial concentrations in all cultures except Entodinium caudatum, in which the overall mean concentration was greater.

Modeling the distribution of ciliate protozoa in the reticulo-rumen using linear programming.

The results from the model application using the contrasting diets of increased forage content versus increased starch content indicate that the majority of rumen protozoa are found in the attached phase, either attached to feed particles or sequestered on the rumen wall, which indicates that the attached protozoal population is a significant component of the total rumen Protozoal community.
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