Evaluation of solute binding to proteins and intra-protein distances from steady state fluorescence measurements.

@article{Alarcon2012EvaluationOS,
  title={Evaluation of solute binding to proteins and intra-protein distances from steady state fluorescence measurements.},
  author={Emilio I Alarcon and Alexis Aspée and Elsa B Abuin and Eduardo A. Lissi},
  journal={Journal of photochemistry and photobiology. B, Biology},
  year={2012},
  volume={106},
  pages={
          1-17
        }
}
Steady state fluorescence measurements, due to their relative simplicity and fast and easy implementation, are one of the most employed techniques for evaluating the non-covalent binding of small molecules to proteins. In the present review we discuss the main characteristics of solute binding and the experimental procedures that can be employed for evaluating both, the efficiency of the process and the number of binding sites. It is also discussed the possibility of determining the distance… CONTINUE READING

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