Evaluation of solubilizing methods of the egg envelope of the fish, Oryzias latipes, and partial determination of amino acid sequence of its subunit protein, ZI-3.

@article{Sugiyama1996EvaluationOS,
  title={Evaluation of solubilizing methods of the egg envelope of the fish, Oryzias latipes, and partial determination of amino acid sequence of its subunit protein, ZI-3.},
  author={Hiroshi Sugiyama and Kousaku Murata and Ichiro Iuchi and Keiko Yamagami},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={1996},
  volume={114 1},
  pages={27-33}
}
The inner layer of most teleostean egg envelopes, especially those after hardening, is almost insoluble in ordinary solvent, and therefore the inner layer of only the unhardened egg envelope has been subjected to solubilization with some potent solvents. We comparatively evaluated the methods of solubilization of the inner layer of egg envelope of medaka, Oryzias latipes, with SDS, urea and guanidium chloride (GuHCI). Analysis of the solubilized samples by SDS-polyacrylamide gel electrophoresis… CONTINUE READING
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