Evaluation of sensitivity of multiplex PCR for Detection of Mycobacterium tuberculosis and Pneumocystis jirovecii in clinical samples.

Abstract

A multiplex PCR assay for the simultaneous detection of Mycobacterium tuberculosis and Pneumocystis jirovecii was developed using IS6110-based detection for M. tuberculosis and mitochondrial large-subunit (mtLSU) rRNA gene detection for P. jirovecii. Ninety-five pulmonary blinded samples were examined using the developed multiplex PCR assay, and the results were compared with those obtained by the single nested PCRs targeting IS6110 for M. tuberculosis and mtLSU rRNA for P. jirovecii. Of the 95 pulmonary samples tested, the multiplex nested PCR developed here could detect 36 cases of M. tuberculosis infection, 35 cases of P. jirovecii infection, and 17 cases of M. tuberculosis and P. jirovecii coinfections. The sensitivities of the multiplex nested PCR in detecting M. tuberculosis and P. jirovecii were 92.1% and 81.4%, respectively, whereas the specificities in detecting M. tuberculosis and P. jirovecii were 98.2% and 100%, respectively.

DOI: 10.1128/JCM.00323-10

5 Figures and Tables

Statistics

02004006002011201220132014201520162017
Citations per Year

72 Citations

Semantic Scholar estimates that this publication has 72 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Boondireke2010EvaluationOS, title={Evaluation of sensitivity of multiplex PCR for Detection of Mycobacterium tuberculosis and Pneumocystis jirovecii in clinical samples.}, author={Sirirat Boondireke and Mathirut Mungthin and Peerapan Tan-Ariya and Petchara Boonyongsunchai and Tawee Naaglor and Anan Wattanathum and Sompong Treewatchareekorn and Saovanee Leelayoova}, journal={Journal of clinical microbiology}, year={2010}, volume={48 9}, pages={3165-8} }