Evaluation of reference genes for quantitative real-time PCR in the brain, pituitary, and gonads of songbirds.

@article{ZinzowKramer2014EvaluationOR,
  title={Evaluation of reference genes for quantitative real-time PCR in the brain, pituitary, and gonads of songbirds.},
  author={Wendy M. Zinzow-Kramer and Brent M. Horton and Donna L. Maney},
  journal={Hormones and behavior},
  year={2014},
  volume={66 2},
  pages={267-75}
}
Quantitative real-time PCR (qPCR) is becoming a popular tool for the quantification of gene expression in the brain and endocrine tissues of songbirds. Accurate analysis of qPCR data relies on the selection of appropriate reference genes for normalization, yet few papers on songbirds contain evidence of reference gene validation. Here, we evaluated the expression of ten potential reference genes (18S, ACTB, GAPDH, HMBS, HPRT, PPIA, RPL4, RPL32, TFRC, and UBC) in brain, pituitary, ovary, and… CONTINUE READING
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