Evaluation of reference genes for quantitative PCR analysis of mouse lymphocytes.

@article{Albershardt2012EvaluationOR,
  title={Evaluation of reference genes for quantitative PCR analysis of mouse lymphocytes.},
  author={Tina Chang Albershardt and Brian M Iritani and Alanna Ruddell},
  journal={Journal of immunological methods},
  year={2012},
  volume={384 1-2},
  pages={196-9}
}
Normalization to a reference gene is the method of choice for quantitative PCR analysis. The stability of reference genes is critical for accurate gene expression analysis, as significant variations in reference gene expression can alter experimental results and conclusions. In this study, we evaluated the expression stability of five commonly used reference genes found in mouse lymphocytes. Using NormFinder and BestKeeper algorithms, we consistently show that ubiquitin C (Ubc) is the optimal… CONTINUE READING