Evaluation of redox state of isolated perfused rat lung.

@article{Fisher1976EvaluationOR,
  title={Evaluation of redox state of isolated perfused rat lung.},
  author={Aron B. Fisher and L Furia and Britton Chance},
  journal={The American journal of physiology},
  year={1976},
  volume={230 5},
  pages={
          1198-1204
        }
}
The metabolic responsiveness of lung tissue to inhibition of oxidative metabolism was determined by measurement of the redox state of the isolated perfused and ventilated rat lung. Changes in redox state were evaluated by fluorescence from the lung surface at wavelengths suitable for reduced pyridine nucleotides and by measurement of the ratios of redox couples in rapidly frozen lung tissue. Maximal change of redox state was observed during ventilation with carbon monoxide; surface fluorescence… 

Fluorescence spectroscopy and cryoimaging of rat lung tissue mitochondrial redox state

The utility of optical cryoimaging and fluorometry to evaluate tissue redox state of the mitochondrial metabolic coenzymes NADH and FAD in intact rat lungs is demonstrated.

Surface Fluorescence Studies of Tissue Mitochondrial Redox State in Isolated Perfused Rat Lungs

The capacity of fluorometry to detect a change in mitochondrial redox state in isolated perfused lungs is demonstrated, and the potential of fluorometers for use in in vivo experiments to extract a sensitive measure of lung tissue health in real-time is suggested.

Optical studies of tissue mitochondrial redox in isolated perfused rat lungs

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Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress.

In KCN perfused lungs the respiratory chain was more reduced, whereas hyperoxia and IR lung tissue have a more oxidized respiratory chain than control lung tissue, consistent with previously measured mitochondrial dysfunction in both hyperoxic and IR lungs.

Optical studies of oxidative stress in lung tissue: Rodent models

In KCN perfused lungs the respiratory chain was more reduced, whereas hyperoxic and IR lung tissue have a more oxidized respiratory chain than control lung tissue, consistent with previously measured mitochondrial dysfunction in both hyperoxia and IR lungs.

Novel Flurometric Tool to Assess Mitochondrial Redox State of Isolated Perfused Rat Lungs After Exposure to Hyperoxia

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Lung as a model for evaluation of critical intracellular PO2 and PCO.

Results with an intact organ confirm previous data with subcellular systems showing a high affinity of the mitochondrial respiratory chain for O2, and indicate that the metabolic changes of hypoxia do not occur until intracellular PO2 approaches 1 mmHg or the CO/O2 exceeds 10.

Intermediary metabolism of the lung.

  • A. Fisher
  • Biology, Medicine
    Environmental health perspectives
  • 1984
The lung is a metabolically active organ engaged in secretion, clearance and other maintenance functions that require reducing potential, energy and substrates for biosynthesis, which represents the major fuel utilized by lung tissues.

Lactate and regulation of lung glycolytic rate.

It is indicated that lactate influences the rate of glycolysis in the normal lung through its utilization as a substrate for mitochondrial metabolism during anoxia, changes in the lung redox state with lactate are a major determinant of the Glycolytic rate.

The effect of exogenous substrate concentrations on true and apparent metabolism of hyperpolarized pyruvate in the isolated perfused lung

The level of hyperpolarized lactate signal in the lung is primarily determined by the rate at which NAD+ is reduced to NADH, and although weak dependences on other factors are predicted, the modeled NAD+ reduction rate is largely governed by the intracellular lactate pool size.