Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy.

@article{Martnez2006EvaluationOR,
  title={Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy.},
  author={Alejandra N{\'o}brega Mart{\'i}nez and Constança Fel{\'i}cia De Paoli de Carvalho Britto and Jos{\'e} Augusto da Costa Nery and Elizabeth Pereira Sampaio and M{\'a}rcia Rodrigues Jardim and Euzenir Nunes Sarno and Milton Oz{\'o}rio Moraes},
  journal={Journal of clinical microbiology},
  year={2006},
  volume={44 9},
  pages={3154-9}
}
In spite of the decrease in the number of registered leprosy patients, the number of new cases diagnosed each year (400,000) has remained essentially unchanged. Leprosy diagnosis is difficult due to the low sensitivity of current methodologies to identify new cases. In this study, conventional and TaqMan real-time PCR assays for detection of Mycobacterium leprae DNA were compared to current classification based on clinical, bacteriological, and histological evaluation. M. leprae DNA was… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 39 extracted citations

Similar Papers

Loading similar papers…