Evaluation of ram semen motility by a swim-up technique.

The turbidity formed as ram spermatozoa swam up into a clear medium was measured using a colorimeter and this was used as an objective index of sperm motility. Wavelengths of 470-475 nm yielded higher optical densities (OD) than did 520-640 nm. The recommended distance between the light beam and semen-medium interface, and operation temperature were 5 mm… CONTINUE READING