Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children.

@article{Kuypers2004EvaluationOQ,
  title={Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children.},
  author={Jane Kuypers and Nancy L. Wright and Rhoda Morrow},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2004},
  volume={31 2},
  pages={123-9}
}
BACKGROUND Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract morbidity in young children and immunosuppressed patients. OBJECTIVES To rapidly and accurately quantify and subtype RSV in respiratory samples, we developed and evaluated two real-time RT-PCR assays. STUDY DESIGN A quantitative assay was designed using primers for a consensus region of the matrix protein gene and a subtype-specific assay for RSV-A and RSV-B detection was designed using primers for the… CONTINUE READING

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