Evaluation of putative internal reference genes for gene expression normalization in Nannochloropsis sp. by quantitative real-time RT-PCR.

@article{Cao2012EvaluationOP,
  title={Evaluation of putative internal reference genes for gene expression normalization in Nannochloropsis sp. by quantitative real-time RT-PCR.},
  author={Shaona Cao and Xiaowen Zhang and Naihao Ye and Xiao Fan and Shanli Mou and Dong Xu and Chengwei Liang and Yitao Wang and Wenqi Wang},
  journal={Biochemical and biophysical research communications},
  year={2012},
  volume={424 1},
  pages={118-23}
}
Quantitative real-time reverse transcription PCR (RT-qPCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. To date, few studies on reference genes have been undertaken for Nannochloropsis sp. In this study, 12 potential reference genes were evaluated for their expression stability using the geNorm and NormFinder statistical algorithms by RT-qPCR. The results showed that the best reference genes differed… CONTINUE READING

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