Evaluation of polyamidoamine dendrimer/alpha-cyclodextrin conjugate (generation 3, G3) as a novel carrier for small interfering RNA (siRNA).

@article{Tsutsumi2007EvaluationOP,
  title={Evaluation of polyamidoamine dendrimer/alpha-cyclodextrin conjugate (generation 3, G3) as a novel carrier for small interfering RNA (siRNA).},
  author={Toshihito Tsutsumi and Fumitoshi Hirayama and Kaneto Uekama and Hidetoshi Arima},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  year={2007},
  volume={119 3},
  pages={349-59}
}
As the first step toward an evaluation of the potential use of the PAMAM dendrimer (G3) conjugate with alpha-cyclodextrin (alpha-CDE) for a small interfering RNA (siRNA) carrier, the ternary complexes of alpha-CDE or the transfection reagents such as Lipofactamine 2000 (L2), TransFast (TF) and Lipofectin (LF) with plasmid DNA (pDNA) and siRNA were prepared, and their RNAi effects, cytotoxicity, physicochemical properties and intracellular distribution were compared. Here the pGL2 control vector… CONTINUE READING