Evaluation of nephrotoxicity in vitro using a suspension of highly purified porcine proximal tubular cells and characterization of the cells in primary culture.

@article{Kruidering1994EvaluationON,
  title={Evaluation of nephrotoxicity in vitro using a suspension of highly purified porcine proximal tubular cells and characterization of the cells in primary culture.},
  author={Marieke Kruidering and D. H. Maasdam and Frans A. Prins and Emile de Heer and Gerard J. Mulder and J. Fred Nagelkerke},
  journal={Experimental nephrology},
  year={1994},
  volume={2 6},
  pages={
          324-44
        }
}
Proximal tubular cells (PTC) were isolated from porcine kidney by collagenase treatment, subsequently purified on a discontinuous density gradient and finally cultured. Porcine PTC (PPTC) in primary culture expressed keratin, characteristics of epithelia and brush border specific glycoproteins (FX1A). In addition, vimentin was present. All cells were negative for the endothelial marker pal-E. Less than 0.1% expressed the Tamm-Horsfall protein, characteristic of the distal tubule, while less… CONTINUE READING

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