Evaluation of monooxygenase induction as a means of enhancing the yield of the rat liver cytochrome P-450 isoenzyme with high affinity for cimetidine.

The binding of cimetidine to liver microsomes prepared from untreated rats and rats pretreated with phenobarbitone or 3-methylcholanthrene has been investigated by difference spectroscopy and equilibrium partition methods. In M/15 phosphate buffer, pH 7.9, microsomes from each group of rats yielded markedly biphasic spectral binding curves, which have been… CONTINUE READING