Evaluation of monomethoxypolyethyleneglycol derivatized superoxide dismutase in blood and evidence for its binding to blood cells.

A method to evaluate in blood free superoxide dismutase (SOD) and SOD covalently bound to monomethoxypolyethylene glycol (MPEG-5000) is reported. The method takes advantage of gel filtration and cationic exchange chromatography to remove substances present in plasma which can interfere with the SOD enzymatic assay. Using this methodology, it was… (More)