Evaluation of mobile phase, ion pairing, and temperature influence on an HILIC-MS/MS method for L-arginine and its dimethylated derivatives detection.

@article{Paglia2008EvaluationOM,
  title={Evaluation of mobile phase, ion pairing, and temperature influence on an HILIC-MS/MS method for L-arginine and its dimethylated derivatives detection.},
  author={Giuseppe Paglia and Oceania D'Apolito and Filomena Tricarico and Daniela Garofalo and Gaetano Corso},
  journal={Journal of separation science},
  year={2008},
  volume={31 13},
  pages={2424-9}
}
Asymmetric N(G),-N(G)-dimethylarginine (ADMA) increases in diseases such as renal failure, diabetes mellitus, and hypercholesterolemia. The feasibility and utility of a hydrophilic interaction chromatography (HILIC) method for the separation of free L-arginine (Arg), ADMA, and symmetric N(G),-N(G')-dimethylarginine (SDMA) on a typical silica column were explored and the impact of some experimental parameters on the chromatographic behavior of these analytes was investigated. The effect of water… CONTINUE READING