Evaluation of methods used to concentrate and detect hepatitis A virus in water samples.

@article{Villar2006EvaluationOM,
  title={Evaluation of methods used to concentrate and detect hepatitis A virus in water samples.},
  author={Livia Melo Villar and V S de Paula and Leonardo Diniz-Mendes and Elisabeth Lampe and Ana Maria Coimbra Gaspar},
  journal={Journal of virological methods},
  year={2006},
  volume={137 2},
  pages={169-76}
}
Two adsorption-elution concentration methods, both involving negatively charged membranes, were evaluated in order to monitor hepatitis A virus (HAV) contamination in tap, river, mineral and coastal water samples: elution with urea-arginine phosphate buffer/reconcentration with magnesium chloride (method 1); and sodium hydroxide elution/reconcentration with a commercial concentrator (method 2). Nested (qualitative) reverse transcriptase PCR (RT-PCR) and real-time (quantitative) RT-PCR were used… CONTINUE READING