Evaluation of metals in a defined medium for Pichia pastoris expressing recombinant beta-galactosidase.

@article{Plantz2007EvaluationOM,
  title={Evaluation of metals in a defined medium for Pichia pastoris expressing recombinant beta-galactosidase.},
  author={Bradley A. Plantz and Kenneth W. Nickerson and Stephen D. Kachman and Vicki L. Schlegel},
  journal={Biotechnology progress},
  year={2007},
  volume={23 3},
  pages={687-92}
}
Culture growth and recombinant protein yield of the Pichia pastoris GS115 methanol utilization positive system were studied in response to the types and levels of metals present in the growth medium and the supplemental salts typically used for these fermentations. Magnesium and zinc were both required to support cell growth but at significantly reduced levels compared to the control. However, supplementation with calcium, cobalt, iron, manganese, iodine, boron, and molybdenum were not required… CONTINUE READING