Evaluation of membrane filter methods for DNA-DNA hybridization


Three membrane filter methods for DNA hybridization were compared. In all of them filter-fixed DNA is spontaneously released during incubation. The effect depends largely on the temperature: 40 to 85% of DNA is released at 70 to 80 C; at higher temperatures most of the DNA is released and heterologous DNA relatedness values are unreliable. Only the… (More)
DOI: 10.1007/BF02069048


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@article{Ley1970EvaluationOM, title={Evaluation of membrane filter methods for DNA-DNA hybridization}, author={Jessica Ley and R. Tijtgat}, journal={Antonie van Leeuwenhoek}, year={1970}, volume={36}, pages={461-474} }