Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.

@article{Mellmann2008EvaluationOM,
  title={Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.},
  author={Alexander Mellmann and Joann Cloud and Thomas Maier and Ursula Keckevoet and I Ramminger and Peter Iwen and John Dunn and Gerri S. Hall and Deborah E Wilson and Patrick A. Lasala and Markus Kostrzewa and Dag Harmsen},
  journal={Journal of clinical microbiology},
  year={2008},
  volume={46 6},
  pages={1946-54}
}
Nonfermenting bacteria are ubiquitous environmental opportunists that cause infections in humans, especially compromised patients. Due to their limited biochemical reactivity and different morphotypes, misidentification by classical phenotypic means occurs frequently. Therefore, we evaluated the use of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) for species identification. By using 248 nonfermenting culture collection strains composed of 37 genera… CONTINUE READING