Evaluation of maleimide derivative of DOTA for site-specific labeling of recombinant affibody molecules.

@article{Ahlgren2008EvaluationOM,
  title={Evaluation of maleimide derivative of DOTA for site-specific labeling of recombinant affibody molecules.},
  author={S. S. Ahlgren and Anna Orlova and Daniel Rosik and Mattias Sandstr{\"o}m and A. -K. Sj{\"o}berg and Barbro Baastrup and Olof Widmark and Gunilla Fant and Joachim Feldwisch and Vladimir Tolmachev},
  journal={Bioconjugate chemistry},
  year={2008},
  volume={19 1},
  pages={235-43}
}
Affibody molecules are a new class of small (7 kDa) scaffold affinity proteins, which demonstrate promising properties as agents for in vivo radionuclide targeting. The Affibody scaffold is cysteine-free and therefore independent of disulfide bonds. Thus, a single thiol group can be engineered into the protein by introduction of one cysteine. Coupling of thiol-reactive bifunctional chelators can enable site-specific labeling of recombinantly produced Affibody molecules. In this study, the use… CONTINUE READING
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