Evaluation of lymphocyte proliferation by immunohistochemistry in the local lymph node assay.

@article{BoussiquetLeroux1995EvaluationOL,
  title={Evaluation of lymphocyte proliferation by immunohistochemistry in the local lymph node assay.},
  author={Christine Boussiquet-Leroux and G Durand-Cavagna and Kiel Herlin and Daniel Holder},
  journal={Journal of applied toxicology : JAT},
  year={1995},
  volume={15 6},
  pages={465-75}
}
A modified version of the local lymph node assay (LLNA) is presented, using bromodeoxyuridine to label proliferating lymphocytes. Cell counting is done on mid-sagittal sections of individual nodes under light microscopy. Two irritants (sodium lauryl sulfate and salicylic acid), four allergens of various sensitizing potential (potassium dichromate, 4-chloroaniline, neomycin sulfate and nickel sulfate) and one chemical of unknown sensitizing potential (ethyl 3-aminobenzoate) were tested either in… CONTINUE READING