• Corpus ID: 8767042

Evaluation of loop-mediated isothermal amplification method for detecting enterotoxin A gene of Staphylococcus aureus in pork.

  title={Evaluation of loop-mediated isothermal amplification method for detecting enterotoxin A gene of Staphylococcus aureus in pork.},
  author={Theeranan Suwanampai and Kobchai Pattaragulvanit and Preprame Pattanamahakul and Orasa Sutheinkul and Kazuhisa Okada and Takeshi Honda and Jiraporn Thaniyavarn},
  journal={The Southeast Asian journal of tropical medicine and public health},
  volume={42 6},
Staphylococcus aureus is an important food-borne pathogen of humans due to ingestion of food containing enterotoxigenic strains. We evaluated the detection of S. aureus enterotoxin A gene (sea) using a loop-mediated isothermal DNA amplification (LAMP) method. Specificity of LAMP method was comparable to that of conventional PCR. Both methods correctly identified sea and did not detect 14 other non-Staphylococcus strains. Limit of detections of LAMP assay for direct detection of S. aureus was 10… 
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