Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map

@article{Sankar2001EvaluationOI,
  title={Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map},
  author={Ajeet A Sankar and Graham A. Moore},
  journal={Theoretical and Applied Genetics},
  year={2001},
  volume={102},
  pages={206-214}
}
Abstract Inter-simple sequence repeat (ISSR) analysis was evaluated for its usefulness in generating markers to extend the genetic linkage map of Citrus using a backcross population previously mapped with restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and isozyme markers. ISSR markers were obtained through the simple technique of PCR followed by analysis on agarose gels, using simple sequence repeat (SSR) primers. Optimization of reaction conditions was… CONTINUE READING

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