Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity.

@article{Kanemura2002EvaluationOI,
  title={Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity.},
  author={Yonehiro Kanemura and Hidehiro Mori and Satoshi Kobayashi and Omedul Islam and Eri Kodama and Atsuyo Yamamoto and Youko Nakanishi and Norio Arita and Masanori Yamasaki and Hideyuki Okano and Masayuki Hara and Jun Miyake},
  journal={Journal of neuroscience research},
  year={2002},
  volume={69 6},
  pages={
          869-79
        }
}
To scale up human neural stem/progenitor cell (NSPC) cultures for clinical use, we need to know how long these cells can live ex vivo without losing their ability to proliferate and differentiate; thus, a convenient method is needed to estimate the proliferative activity of human NSPCs grown in neurosphere cultures, as direct cell counting is laborious and potentially inaccurate. Here, we isolated NSPCs from human fetal forebrain and prepared neurosphere cultures. We determined the number of… CONTINUE READING
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