Evaluation of immunomagnetic separation and PCR for the detection of Escherichia coli O157 in animal feces and meats.

@article{Islam2006EvaluationOI,
  title={Evaluation of immunomagnetic separation and PCR for the detection of Escherichia coli O157 in animal feces and meats.},
  author={Md. Aminul Islam and Annet E. Heuvelink and Kaisar Ali Talukder and Marcel H. Zwietering and Enne de Boer},
  journal={Journal of food protection},
  year={2006},
  volume={69 12},
  pages={2865-9}
}
Series of animal feces and meat samples artificially contaminated with strains of Escherichia coli O157 isolated from different sources were tested by both an immunomagnetic separation (IMS)-based method and a PCR method using primers specific for a portion of the rfbE gene of E. coli O157. IMS is laborious and time consuming but ends up with the isolation of the pathogen. PCR is fast and less laborious, but it can only be used for screening purposes, so a further culture step is required to… CONTINUE READING

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