Evaluation of immobilized metal-ion affinity chromatography for the fractionation of natural Cu complexing ligands.

@article{Paunovic2005EvaluationOI,
  title={Evaluation of immobilized metal-ion affinity chromatography for the fractionation of natural Cu complexing ligands.},
  author={Irena Paunovic and Rainer Schulin and Bernd Nowack},
  journal={Journal of chromatography. A},
  year={2005},
  volume={1100 2},
  pages={176-84}
}
An immobilized metal-ion affinity chromatography (IMAC) method has been developed and validated for the separation of copper complexing ligands from soil solution. We first investigated the retention behavior of simple model ligands on the IMAC column and found that the ability to form ternary complexes of the structure Cu-IDA-ligand was the dominant factor influencing ligand retention on the IMAC column. The logK value of the Cu-complex was found to have only a minor influence on the retention… CONTINUE READING