Evaluation of high performance liquid chromatography for routine estimation of haemoglobins A2 and F.

@article{Tan1993EvaluationOH,
  title={Evaluation of high performance liquid chromatography for routine estimation of haemoglobins A2 and F.},
  author={Guat Bee Tan and T C Aw and Robin Dunstan and Soon Hong Lee},
  journal={Journal of clinical pathology},
  year={1993},
  volume={46 9},
  pages={852-6}
}
AIMS To compare high performance liquid chromatography (HPLC) with conventional methods for the estimation of blood haemoglobin A2 (HbA2) and haemoglobin F (HbF) concentrations in routine thalassemia screening. METHODS An HPLC system (the VARIANT Hemoglobin Testing System) was tested for precision and reproducibility in the measurement of HbA2 and HbF, and reference ranges were obtained for a local healthy adult population. HPLC was compared with column anion exchange chromatography for HbA2… CONTINUE READING