Evaluation of glucocorticoid-induced growth hormone gene expression in chicken embryonic pituitary cells using a novel in situ mRNA quantitation method.

@article{Bossis2003EvaluationOG,
  title={Evaluation of glucocorticoid-induced growth hormone gene expression in chicken embryonic pituitary cells using a novel in situ mRNA quantitation method.},
  author={Ioannis Bossis and Tom E. Porter},
  journal={Molecular and cellular endocrinology},
  year={2003},
  volume={201 1-2},
  pages={13-23}
}
We reported that corticosterone (CORT) can induce differentiation of growth hormone (GH) cells in vitro and in vivo during chick embryonic development. In the present study, a quantitative in situ hybridization plate assay (ISHPA) for GH mRNA was developed and used to assess the mechanism of glucocorticoid-induced GH gene expression directly in cell culture plates. Embryonic pituitary cells were treated with GH-releasing hormone (GHRH) alone, CORT alone and GHRH and CORT in combination. CORT… CONTINUE READING

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