Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR.

@article{Haugland2010EvaluationOG,
  title={Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR.},
  author={Richard A. Haugland and Manju Varma and Mano Sivaganesan and Catherine A. Kelty and Lindsay Peed and Orin C. Shanks},
  journal={Systematic and applied microbiology},
  year={2010},
  volume={33 6},
  pages={348-57}
}
Molecular methods for quantifying defined Bacteroidales species from the human gastrointestinal tract may have important clinical and environmental applications, ranging from diagnosis of infections to fecal source tracking in surface waters. In this study, sequences from the V2 region of the small subunit ribosomal RNA gene were targeted in the development of qPCR assays to quantify DNA from six Bacteroides and one Prevotella species. In silico and experimental analyses suggested that each of… CONTINUE READING

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