Evaluation of four pretreatment procedures for MALDI-TOF MS yeast identification in the routine clinical laboratory.

@article{Cassagne2013EvaluationOF,
  title={Evaluation of four pretreatment procedures for MALDI-TOF MS yeast identification in the routine clinical laboratory.},
  author={Carole Cassagne and Anne-Laure Cella and Pierre Suchon and Anne-C{\'e}cile Normand and St{\'e}phane Ranque and Renaud Piarroux},
  journal={Medical mycology},
  year={2013},
  volume={51 4},
  pages={371-7}
}
MALDI-TOF MS-based yeast identification requires a pretreatment step for which four are described in the literature, i.e., direct smear, fast formic acid and two complete formic acid/acetonitrile extractions. In this study we compared the impact of these procedures on the performance of MALDI-TOF MS-based yeast identification of samples from colonies grown on Sabouraud or chromogenic media. A total of 103 yeast isolates recovered from clinical samples were identified in parallel using the four… CONTINUE READING

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