Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH‐SY5Y

@article{Sebasti2003EvaluationOF,
  title={Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH‐SY5Y},
  author={Jordi Ponce Sebasti{\`a} and Rosa Crist{\`o}fol and Manuela Mart{\'i}n and Eduard Rodrı́guez-Farr{\'e} and Coral Sanfeliu},
  journal={Cytometry Part A},
  year={2003},
  volume={51A}
}
Reduced glutathione (GSH) protects cells against oxidative injury and maintains a range of vital functions. To study GSH content in human neuronal cell cultures, thiol‐sensitive fluorescent techniques requiring a small number of cells may be of great value, but their GSH specificity has not been established in these cells. 
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105 Citations
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21
Methods Citations
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105 Citations

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Citation Type

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TLDR
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TLDR
Although GSH modulation influences MeHg-induced toxicity, the difference in the content of GSH in cortical and cerebellar cultures fails to account for the increased ROS production in cerebellAR cultures. Expand
A new method of quantifying glutathione levels in freshly isolated single superfused rat cardiomyocytes.
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Functional Induction of the Cystine-Glutamate Exchanger System Xc - Activity in SH-SY5Y Cells by Unconjugated Bilirubin
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It is suggested that bilirubin can modulate the gluthathione levels in neuroblastoma cells through the induction of the System Xc −, and this renders the cell less prone to oxidative damage. Expand
Determination of glutathione and glutathione disulfide in biological samples: an in-depth review.
TLDR
High-performance liquid chromatography, capillary electrophoresis and gas chromatography (as techniques with a separation step) with photometric, fluorimetric, electrochemical and mass spectrometric detection are discussed, stress being laid on novel approaches. Expand
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Reduced glutathione (GSH) protects cells against injury by oxidative stress and maintains a range of vital functions. In vitro cell cultures have been used as experimental models to study the role of… Expand
γ-Tocotrienol does not substantially protect DS neurons from hydrogen peroxide-induced oxidative injury
TLDR
It is suggested that γT3 pre-treatment are not sufficient to protect DS neurons from H2O2-induced oxidative assault, instead induced the apoptosis process. Expand
Loss of glutathione homeostasis associated with neuronal senescence facilitates TRPM2 channel activation in cultured hippocampal pyramidal neurons
TLDR
GSH plays a physiologically relevant role in the regulation of TRPM2 currents in hippocampal pyramidal neurons, and this interaction may play an important role in aging and neurological diseases associated with depletion of GSH. Expand
Recent developments in methods intracellulary localizing glutathione within plant tissues and cells (a minireview)
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A specific antibody, which recognizes t-GSH, was used in order to demonstrate the distribution of glutathione in different cell compartments for light and electron microscopical investigations. Expand
Comparison of glutathione levels measured using optimized monochlorobimane assay with those from ortho-phthalaldehyde assay in intact cells.
TLDR
It is concluded that measuring glutathione using monochlorobimane with the here-described optimized evaluation of fluorescence signal could be a simple and useful method for routine and rapid assessment of glutATHione within intact cells in large numbers of samples. Expand
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