Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH‐SY5Y

@article{Sebasti2003EvaluationOF,
  title={Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH‐SY5Y},
  author={J. Sebasti{\`a} and R. Crist{\`o}fol and M. Mart{\'i}n and E. Rodrı́guez-Farr{\'e} and C. Sanfeliu},
  journal={Cytometry Part A},
  year={2003},
  volume={51A}
}
  • J. Sebastià, R. Cristòfol, +2 authors C. Sanfeliu
  • Published 2003
  • Biology, Medicine
  • Cytometry Part A
    • Reduced glutathione (GSH) protects cells against oxidative injury and maintains a range of vital functions. To study GSH content in human neuronal cell cultures, thiol‐sensitive fluorescent techniques requiring a small number of cells may be of great value, but their GSH specificity has not been established in these cells. 
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    References

    SHOWING 1-10 OF 32 REFERENCES
    Characterization of Hydrogen Peroxide Toxicity in Cultured Rat Forebrain Neurons
    • 77
    Glutathione levels in primary glial cultures: Monochlorobimane provides evidence of cell type‐specific distribution
    • 86
    Evaluation of glutathione‐sensitive fluorescent dyes in cortical culture
    • 89
    Direct evidence for glutathione as mediator of apoptosis in neuronal cells.
    • 33
    Glutathione depletion and neuronal cell death: the role of reactive oxygen intermediates and mitochondrial function
    • 174
    Synthesis of the Antioxidant Glutathione in Neurons: Supply by Astrocytes of CysGly as Precursor for Neuronal Glutathione
    • 516
    • PDF
    Quantitative analysis of cellular glutathione by flow cytometry utilizing monochlorobimane: some applications to radiation and drug resistance in vitro and in vivo.
    • 251
    Influence of glutathione S-transferases on cellular glutathione determination by flow cytometry using monochlorobimane.
    • 78
    Evaluation of methods for measuring cellular glutathione content using flow cytometry.
    • 227
    Metabolism and functions of glutathione in brain
    • R. Dringen
    • Biology, Medicine
    • Progress in Neurobiology
    • 2000
    • 1,455
    • PDF