Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH‐SY5Y

@article{Sebasti2003EvaluationOF,
  title={Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH‐SY5Y},
  author={Jordi Ponce Sebasti{\`a} and Rosa Crist{\`o}fol and Manuela Mart{\'i}n and Eduard Rodrı́guez-Farr{\'e} and Coral Sanfeliu},
  journal={Cytometry Part A},
  year={2003},
  volume={51A}
}
Reduced glutathione (GSH) protects cells against oxidative injury and maintains a range of vital functions. To study GSH content in human neuronal cell cultures, thiol‐sensitive fluorescent techniques requiring a small number of cells may be of great value, but their GSH specificity has not been established in these cells. 
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Reduced glutathione (GSH) protects cells against injury by oxidative stress and maintains a range of vital functions. In vitro cell cultures have been used as experimental models to study the role ofExpand
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It is suggested that γT3 pre-treatment are not sufficient to protect DS neurons from H2O2-induced oxidative assault, instead induced the apoptosis process. Expand
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TLDR
GSH plays a physiologically relevant role in the regulation of TRPM2 currents in hippocampal pyramidal neurons, and this interaction may play an important role in aging and neurological diseases associated with depletion of GSH. Expand
Recent developments in methods intracellulary localizing glutathione within plant tissues and cells (a minireview)
TLDR
A specific antibody, which recognizes t-GSH, was used in order to demonstrate the distribution of glutathione in different cell compartments for light and electron microscopical investigations. Expand
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TLDR
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