Evaluation of fluorescence spectroscopy methods for mapping melted regions of DNA along the transcription pathway.

@article{Martin2003EvaluationOF,
  title={Evaluation of fluorescence spectroscopy methods for mapping melted regions of DNA along the transcription pathway.},
  author={Craig T. Martin and Andrea {\'U}jv{\'a}ri and C Liu},
  journal={Methods in enzymology},
  year={2003},
  volume={371},
  pages={
          13-33
        }
}
Publisher Summary This chapter evaluates the fluorescence Spectroscopy technique for mapping melted regions of DNA along the transcription pathway. Fluorescent base analogs provide a relatively non-perturbing probe of very local structural changes within the DNA and/or RNA. They can be used to map melted bubbles in various static states of transcription, either the initially bound promoter complexes, or through walking experiments, in stalled complexes approximating dynamic movement along the… CONTINUE READING

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