Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery.

@article{Lo2004EvaluationOF,
  title={Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery.},
  author={M Lo and Ann Aulabaugh and Guixian Jin and Rebecca A. Cowling and Jonathan A. Bard and Michael Malamas and George Ellestad},
  journal={Analytical biochemistry},
  year={2004},
  volume={332 1},
  pages={153-9}
}
The fluorescence-based thermal shift assay is a general method for identification of inhibitors of target proteins from compound libraries. Using an environmentally sensitive fluorescent dye to monitor protein thermal unfolding, the ligand-binding affinity can be assessed from the shift of the unfolding temperature (Delta Tm) obtained in the presence of ligands relative to that obtained in the absence of ligands. In this article, we report that the thermal shift assay can be conducted in an… CONTINUE READING
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