[Evaluation of flow-through immunoassay for rapid detection of influenza A and B viruses].

Abstract

We evaluated a flow-through immunoassay for rapid detection of influenza A and B viral antigens, RapidTesta FLU AB (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), by using 507 specimens collected from patients with influenza-like symptoms during the 2002/2003 influenza season in Japan. The specimens consisted of 239 nasal swabs and 268 nasal aspirates; 374 specimens were collected from pediatric patients (under 16 years of age) and 133 from adult patients. RapidTesta FLU AB was compared with cell culture and nested reverse transcription-polymerase chain reaction (RT-PCR). Cell culture detected influenza virus from 66.7% of the 507 specimens (influenza AH3: 44.0%, B: 22.7%). For nasal swabs, it had a sensitivity of 81.9% (77/94), a specificity of 97.9% (142/145) and an efficiency of 91.6% (219/239) for influenza A virus as well as a sensitivity of 80.0% (52/65), a specificity of 98.3% (171/174) and an efficiency of 93.3% (223/239) for influenza B. For nasal aspirates, RapidTesta FLU AB had a sensitivity of 83.2% (109/131), a specificity of 98.5% (135/137) and an efficiency of 91.0% (244/268) for influenza A as well as a sensitivity of 82.7% (43/52), a specificity of 97.7% (211/216) and an efficiency of 94.8% (254/268) for influenza B. RapidTesta FLU AB is characterized by high specificity, low false positive rate, and 10-minute detection of influenza virus. These advantages suggest that RapidTesta FLU AB is a useful kit to assist physicians in making a diagnosis of influenza on candidates for antiviral therapy.

Cite this paper

@article{Yamazaki2004EvaluationOF, title={[Evaluation of flow-through immunoassay for rapid detection of influenza A and B viruses].}, author={Masahiko Yamazaki and Keiko Mitamura and Masataka Ichikawa and Kazuhiro Kimura and Osamu Komiyama and Hideaki Shimizu and Chiharu Kawakami and Sumi Watanabe and Mitsunobu Imai and Hideo Cho and Yoshinao Takeuchi}, journal={Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases}, year={2004}, volume={78 9}, pages={865-71} }