Evaluation of five extraction protocols for quantification of endotoxin in metalworking fluid aerosol.

@article{Thorne2003EvaluationOF,
  title={Evaluation of five extraction protocols for quantification of endotoxin in metalworking fluid aerosol.},
  author={Peter S Thorne and Katharine Helen Bartlett and John S. Phipps and Katarina Kulh{\'a}nkov{\'a}},
  journal={The Annals of occupational hygiene},
  year={2003},
  volume={47 1},
  pages={
          31-6
        }
}
OBJECTIVES Occupational exposures to endotoxin-contaminated, water-based metalworking fluids (MWFs) are thought to contribute to cases of respiratory illness. Before occupational exposure limits for endotoxin can be proposed, accuracy and reproducibility of laboratory measurements must be established. The method most commonly used to quantify endotoxin is the Limulus amebocyte lysate (LAL) assay and this is the basis for the American Society for Testing and Materials (ASTM) method E2144-01… CONTINUE READING

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