Evaluation of enzyme-linked immunosorbent assays: a method of data analysis.

@article{Crofts1988EvaluationOE,
  title={Evaluation of enzyme-linked immunosorbent assays: a method of data analysis.},
  author={Nick Crofts and William J Maskill and Ian David Gust},
  journal={Journal of virological methods},
  year={1988},
  volume={22 1},
  pages={51-9}
}
Diagnostic tests are usually evaluated in terms of simple qualitative measures of sensitivity and specificity. When comparing different quantitative assays such as ELISAs, it is often more useful to deal with actual values (sample optical density/cut-off optical density ratio (OD ratio] rather than the qualitative relationship to the cut-off, i.e. positive or negative. This allows for a statistical approach to the questions of sensitivity and specificity. The National HIV Reference Laboratory… CONTINUE READING