Evaluation of enzyme immunoassays and real-time PCR for detecting Shiga toxin-producing Escherichia coli in Southern Alberta, Canada.

Abstract

Two immunoassays (Shiga Toxin Chek and Shiga Toxin Quik Chek) and real-time PCR were used to detect Shiga toxin-producing Escherichia coli. For enriched culture, the sensitivity and specificity of the three methods ranged from 80.0% to 98.2% and 98.0% to 100.0%, respectively. STEC isolates were identified in 2.6% of the 784 samples. 
DOI: 10.1128/JCM.03288-14

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Cite this paper

@article{Chui2015EvaluationOE, title={Evaluation of enzyme immunoassays and real-time PCR for detecting Shiga toxin-producing Escherichia coli in Southern Alberta, Canada.}, author={Linda W Chui and Laura M. Patterson-Fortin and Julie H Kuo and Vincent Li and Valerie F. Boras}, journal={Journal of clinical microbiology}, year={2015}, volume={53 3}, pages={1019-23} }